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References




The following includes selected links and publications describing applications of the pZ Expression System in various fields of basic and applied research. A complete list of citing arcticles can be found at e.g. Google Scholar.

Quantitative control and analysis of gene activities

Basan M et al. (2015). Overflow metabolism in Escherichia coli results from efficient proteome allocation. Nature, 528, 99 (link)

Brewster RC et al. (2014). The Transcription Factor Titration Effect Dictates Level of Gene Expression. Cell,156, 6, 1312 (link)

Mutalik VK et al. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10, 354 (link)

Da Re S,
et al. (2007). Tight Modulation of Escherichia coli Bacterial Biofilm Formation through Controlled Expression of Adhesion Factors. Applied and Environmental Microbiology, 73, 10, 3391 (link)

Le TT
et al. (2006). Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium. Biophys J, 90, 9, 3315 (link)

Golding I & Cox EC (2004). RNA dynamics in live Escherichia coli cells. Proc Natl Acad Sci USA, 101, 31, 11310 (link)

Elowitz MB
et al. (2002). Stochastic Gene Expression in a Single Cell. Science, 297, 5584, 1183 (link)


Independent control of gene activities

Rodrigo G
et al. (2012). De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci USA,109, 38, 15271 (link)

Chubiz LM & Rao CV (2008). Computational design of orthogonal ribosomes. Nucleic Acids Res, 36, 12, 403 (link)

Leandroa J et al. (2006), P Leandro. Co-expression of different subunits of human phenylalanine hydroxylase: Evidence of negative interallelic complementation. Biochimica et Biophysica Acta, 1762, 5, 544 (link)

Kapust RB & Waugh DS (2000). Controlled Intracellular Processing of Fusion Proteins by TEV Protease. Protein Expression and Purification, 19, 2, 312 (link)


Cloning and Expression of toxic or harmful Gene Products

Fan Y et al. (2016). Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27. Extremophyles 1-9
(link)

Kannoly S et al. (2012). Rethinking the evolution of single-stranded RNA (ssRNA) bacteriophages based on genomic sequences and characterizations of two R-plasmid-dependent ssRNA phages, C-1 and Hgal1. J Bacteriol, 194, 18, 5073
(link)

Mok WWK et al. (2010). Decoding toxicity: Deducing the sequence requirements of IbsC, a type I toxin in Escherichia coli. J Biol Chem, 285, 41627
(link)

You L
et al. (2004). Programmed population control by cell–cell communication and regulated killing. Nature, 428, 868 (link)

Grundling A
et al. (2001). Holins kill without warning. Proc Natl Acad Sci USA, 98, 16, 9348-9352 (link)


Screening

Genee HJ et al. (2016). Functional mining of transporters using synthetic selections. Nature Chem Biol, 12, 1015
(link)

Fleck LE et al. (2014). A Screen for and Validation of Prodrug Antimicrobials. Antimicrob. Agents Chemother, 58:3 1410
(link)

Forsberg KJ et al. (2012). The shared antibiotic resistome of soil bacteria and human pathogens. Science, 337, 6098, 1107
(link)

Hsiao A et al. (2006). Vibrio cholerae virulence regulator-coordinated evasion of host immunity. Proc Natl Acad Sci USA, 103, 39, 14542 (link)

Cabantous S
et al. (2005). Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nature Biotech, 23, 1, 102 (link)

Christ D & Winter G (2003). Identification of functional similarities between proteins using directed evolution. Proc Nat Acad Sci USA, 100, 23, 13202 (link)


Transcriptional Cascades/Genetic Switches

Rubens JR et al. (2016). Synthetic mixed-signal computation in living cells. Nature Communications, 7 (link)

Park H
et al. (2010). Interdependence of behavioural variability and responses to small stimuli in bacteria. Nature, 468, 819 (link)

Stricker J
et al. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456, 7221, 516 (link)

Hooshangi S
et al. (2005). Ultrasensitivity and noise propagation in a synthetic transcriptional cascade. Proc Natl Acad Sci USA, 102, 10, 3581 (link)

Kobayashi H
et al. (2004). Programmable cells: Interfacing natural and engineered gene networks. Proc Natl Acad Sci USA, 101, 22, 8414 (link)

Guet CC
et al. (2002). Combinatorial Synthesis of Genetic Networks. Science, 296, 5572, 1466 (link)


Overexpression

Oliver JWK (2012). Cyanobacterial conversion of carbon dioxide to 2,3-butanediol, Proc Natl Acad Sci USA, 110, 4, 1249 (link)

Guillier M & Gottesman S (2006). Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs. Mol Microbiol, 59 (1), 231 (link)

Zhao K,
et al. (2005) The Global Transcriptional Response of Escherichia coli to Induced Sigma32 Protein Involves Sigma32 Regulon Activation Followed by inactivation and Degradation of Sigma32 in Vivo. J Biol Chem, 280, 18, 17758 (link)

Cabantous S
et al. (2005). Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nature Biotech, 23, 1, 102 (link)

Kauth CW
et al. (2003). The Merozoite Surface Protein 1 Complex of Human Malaria Parasite Plasmodium falciparum: Interactions and arrangements of subunits. J Biol Chem, 278, 25, 22257 (link)


Expression in hosts other than E. coli

Nyerges A et al. (2015). A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species. Proc Natl Acad Sci USA, 113, 9 , 2502 (link)

Lathem WW
et al. (2007). A Plasminogen-Activating Protease Specifically Controls the Development of Primary Pneumonic Plague. Science, 315, 5811, 509 (link)

Hsiao A
et al. (2006) Vibrio cholerae virulence regulator-coordinated evasion of host immunity. Proc Natl Acad Sci USA, 103, 39, 14542 (link)

Qian F & Pan W (2002). Construction of a tetR-Integrated Salmonella enterica Serovar Typhi CVD908 Strain That Tightly Controls Expression of the Major Merozoite Surface Protein of Plasmodium falciparum for Applications in Human Vaccine Production. Infect Immun, 70, 4, 2029 (link)


Reviews

Purcell O
et al. (2010). A comparative analysis of synthetic genetic oscillators. Journal of the Royal Society Interface, 7, 52, 1503 (link)

Bertram Rand Hillen W (2008). The application of Tet repressor in prokaryotic gene regulation and expression. Microbial Biotechnol,1 , 1, 2 (link)

Saida F
et al. (2006). Expression of Highly Toxic Genes in E. coli: Special Strategies and Genetic Tools, in: Current Protein and Peptide Science, 7, 1, 47, Bentham Science Publishers (link)

McDaniel R & Weiss R (2005). Advances in synthetic biology: on the path from prototypes to applications. Current Opinion in Biotechnology, 16, 476 (link)

Kaern M
et al. (2003). The engeneering of gene regulatory networks. Annu Rev Biomed Eng, 5,179 (link)