Tools & Reagents |
| Vector | Origin of replication | Resistance | Promoter | Cloning site |
Data sheet (.pdf) |
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| pZS*13luc | pSC101* | Ampicillin | PA1lacO-1 | luciferase | pZS*13luc.pdf | |||||
| pZS*21MCS | pSC101* | Kanamycin | PLtetO-1 | MCS | pZS*21MCS.pdf | |||||
| pZS*21luc | pSC101* | Kanamycin | PLtetO-1 | luciferase | pZS*21luc.pdf | |||||
| pZS*24MCS | pSC101* | Kanamycin | Plac/ara-1 | MCS | pZS*24MCS.pdf | |||||
| pZS*24luc | pSC101* | Kanamycin | Plac/ara-1 | luciferase | pZS*24luc.pdf | |||||
| pZS13luc | pSC101 | Ampicillin | PA1lacO-1 | luciferase | pZS13luc.pdf | |||||
| pZS21MCS | pSC101 | Kanamycin | PLtetO-1 | MCS | pZS21MCS.pdf | |||||
| pZS21luc | pSC101 | Kanamycin | PLtetO-1 | luciferase | pZS21luc.pdf | |||||
| pZA11MCS | p15A | Ampicillin | PLtetO-1 | MCS | pZA11MCS.pdf | |||||
| pZA13luc | p15A | Ampicillin | PA1lacO-1 | luciferase | pZA13luc.pdf | |||||
| pZA14luc | p15A | Ampicillin | Plac/ara-1 | luciferase | pZA14luc.pdf | |||||
| pZA21MCS | p15A | Kanamycin | PLtetO-1 | MCS | pZA21MCS.pdf | |||||
| pZA23MCS | p15A | Kanamycin | PA1lacO-1 | MCS | pZA23MCS.pdf | |||||
| pZA24luc | p15A | Kanamycin | Plac/ara-1 | luciferase | pZA24luc.pdf | |||||
| pZA31MCS | p15A | Chloramphenicol | PLtetO-1 | MCS | pZA31MCS.pdf | |||||
| pZA31luc | p15A | Chloramphenicol | PLtetO-1 | luciferase | pZA31luc.pdf | |||||
| pZA33luc | p15A | Chloramphenicol | PA1lacO-1 | luciferase | pZA33luc.pdf | |||||
| pZA34luc | p15A | Chloramphenicol | Plac/ara-1 | luciferase | pZA34luc.pdf | |||||
| pZA35luc | p15A | Chloramphenicol | PN25tetO-1 | luciferase | pZA35luc.pdf | |||||
| pZE11luc | colE1 | Ampicillin | PLtetO-1 | luciferase | pZE11luc.pdf | |||||
| pZE12MCS | colE1 | Ampicillin | PLlacO-1 | MCS | pZE12MCS.pdf | |||||
| pZE12luc | colE1 | Ampicillin | PLlacO-1 | luciferase | pZE12luc.pdf | |||||
| pZE13MCS | colE1 | Ampicillin | PA1lacO-1 | MCS | pZE13MCS.pdf | |||||
| pZE13luc | colE1 | Ampicillin | PA1lacO-1 | luciferase | pZE13luc.pdf | |||||
| pZE14MCS | colE1 | Ampicillin | Plac/ara-1 | MCS | pZE14MCS.pdf | |||||
| pZE15luc | colE1 | Ampicillin | PN25tetO-1 | luciferase | pZE15luc.pdf | |||||
| pZE21MCS | colE1 | Kanamycin | PLtetO-1 | MCS | pZE21MCS.pdf | |||||
| pZE21luc | colE1 | Kanamycin | PLtetO-1 | luciferase | pZE21luc.pdf | |||||
| pZE22luc | colE1 | Kanamycin | PLlacO-1 | luciferase | pZE22luc.pdf | |||||
| pZE23MCS | colE1 | Kanamycin | PA1lacO-1 | MCS | pZE23MCS.pdf | |||||
| pZE23luc | colE1 | Kanamycin | PA1lacO-1 | luciferase | pZE23luc.pdf | |||||
| pZE24MCS | colE1 | Kanamycin | Plac/ara-1 | MCS | pZE24MCS.pdf | |||||
| pZE24luc | colE1 | Kanamycin | Plac/ara-1 | luciferase | pZE24luc.pdf | |||||
| pZE25luc | colE1 | Kanamycin | PN25tetO-1 | luciferase | pZE25luc.pdf | |||||
| pZE31luc | colE1 | Chloramphenicol | PLtetO-1 | luciferase | pZE31luc.pdf | |||||
| pZE32luc | colE1 | Chloramphenicol | PLlacO-1 | luciferase | pZE32luc.pdf | |||||
| pZE33luc | colE1 | Chloramphenicol | PA1lacO-1 | luciferase | pZE33luc.pdf | |||||
| pZE34MCS | colE1 | Chloramphenicol | Plac/ara-1 | MCS | pZE34MCS.pdf | |||||
| pZE53luc | colE1 | Tetracycline | PA1lacO-1 | luciferase | pZE53luc.pdf |
| Vector | Origin of replication | Resistance | Promoter | Cloning site |
Map/ Sequence (.pdf) |
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| pZS41MCS-Int | pSC101 | Spectinomycin | PLtetO-1 | MCS | pZS41MCS Int.pdf | |||||
| pZS43MCS-Int | pSC101 | Spectinomycin | PA1lacO-1 | MCS | pZS43MCS Int.pdf | |||||
| pZS43MCSlaci-Int | pSC101 | Spectinomycin | PA1lacO-1 | MCS | pZS43MCS laci Int.pdf | |||||
| pZS4Int-laci | pSC101 | Spectinomycin | -- | -- | pZS4Int-laci.pdf | |||||
| pZS4Int-tetR | pSC101 | Spectinomycin | -- | -- | pZS4Int-tetR.pdf | |||||
| pZS4Int-laci/tetR | pSC101 | Spectinomycin | -- | -- | pZS4Int-laci/tetR.pdf |
| Strain | Genotype | Main features | ||
DH5alphaZi |
laciq, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 |
Its high transformation efficiency makes DH5alpha suited for DNA cloning and DNA preparation. Due to its relatively low growth rates it is not widely used for large scale protein production (e.g. by fermentation) |
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| DH5alphaZ1 | laciq, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15 |
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| DH5alphaInt |
pIntAssist (pSC101ts, KnR, PLts-int), deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15 |
DH5alphaInt carries a temperature sensitive plasmid harbouring the Lambda int gene (encoding Integrase) driven by a temperature inducible promoter. It is especially suited for Integrase mediated chromosomal integration of expression units. |
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| C600Z1 |
laciq, PN25-tetR, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21 |
A high transformation efficiency combined with extremly fast growth rates makes C600Z1 a superb candidate for DNA cloning as well as high level protein production. RecA mediated chromosomal integration of DNA-sequences and general transduction, e.g. by P1, is also feasible. |
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| C600Zi |
laciq, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21 |
Upon request, this strain is also available as a certified strain with proven strain history fulfilling all requirements for Good manufactoring practice (GMP) productions. |
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| C600Int |
pIntAssist (pSC101ts, KnR, PLts-int), lacY1, leuB6,mcrB+, supE44, thi-1, thr-1, tonA21 |
C600Int carries a temperature sensitive plasmid harbouring the Lambda int gene (encoding Integrase) driven by a temperature inducible promoter. It is especially suited for Integrase mediated chromosomal integration of expression units. |
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| W3110Z1 |
laciq, PN25-tetR, SpR, IN (rrnD-rrnE)1, rph-1 (see: ATCC 39936) |
This strain is widely used in large scale, commercial protein production processes. Due to its low transformation efficiency it is not suited for DNA cloning. |
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| Kit | Purpose of use | System components | Main features |
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| pZ Lac Basic |
Standard DNA cloning. High-level expression of recombinant proteins. Protein purification. |
Cloning and Expression Vectors: pZE13MCS (pZE13MCS.pdf) pZE13luc (pZE13luc.pdf) Control Expression Vector: pZE13p85 2 Sequencing primers (Forward/Reverse, 1 nMol each) E. coli strain C600Zi |
Vectors contain combinations of the following modules: • Expression module: IPTG-inducible promoter PA1lacO-1 in combination with either MCS or luciferase • Resistance module: Ampicillin • Replication module: colE1 Host strain C600Zi produces constitutively chromosomally encoded Lac Repressor |
| pZ Tet Basic |
Standard DNA cloning. High-level expression of recombinant proteins. Protein purification. |
Cloning and Expression Vectors: pZE15luc (pZE15luc.pdf) Control Expression Vector: pZE15p120 2 Sequencing primers (Forward/Reverse, 1 nMol each) E. coli strain C600Z1 |
Vectors contain com binations of the following modules: • Expression module: Tetracycline-inducible promoter PN25tetO-1 in combination with luciferase • Resistance module: Ampicillin • Replication module: colE1 Host strain C600Z1 produces constitutively chromosomally encoded Lac Repressor and Tet Repressor |
| pZ High Performance |
Quantitative and independent control of up to three different transcription units. Cloning and expression of toxic genes and gene products. Functional analysis of low copy number proteins where a broad window of regulation (up to 80,000 fold) and/or graded expression is required. High-level expression of recombinant proteins |
Cloning and Expression Vectors: pZE21MCS (pZE21MCS.pdf) pZA31luc (pZA31luc.pdf) pZE12luc (pZE12luc.pdf) pZE53luc (pZE53luc.pdf) pZS*24MCS (pZS*24MCS) Control Expression vectors: pZE21p120 and pZE23p85 3 Sequencing primers (2 Forward, 1 Reverse, 1 nMol each) E. coli strains: DH5alphaZ1 W3110Z1 |
Vectors contain combinations of the following modules: • Expression modules: IPTG-inducible promoters PA1lacO-1 and PLlacO-1 IPTG/Arabinose-inducible promoter Plac/ara-1 , Doxycycline-inducible promoter PLtetO-1 combined with either an MCS or luciferase. • Resistance modules: Kanamycin Chloramphenicol Tetracycline • Replication modules: colE1 p15A pSC101* Vector modules are largely interchangeable by unique restriction sites and compatible with pZ BASIC and pZ INTEGRATION. HHost strain DH5alphaZ1 and W3110Z1 produce constitutively chromosomally encoded LacR, TetR, and AraC. |
| pZ Custom | Construction of custom E. coli host strains producing constitutively LacR and/or TetR from chromosomally or episomally located genes. |
Vectors for chromosomal integration or episomal maintenance: pZS4Int-laci (pZS4Int-laci.pdf) pZS4Int-tetR (pZS4Int-tetR.pdf) pZS4Int-laci/tetR (pZS4Int-laci/tetR.pdf) pIntAssist |
Vectors carry genes laci and tetR under the control of constitutive promoters Placiq and PN25, respectively, either separately (pZS4Int-laci and pZS4Int-tetR) or in combination (pZS4Int-laci/tetR). • Resistance module: Spectinomycin • Replication module: pSC101 All vectors can be propagated episomally or can be integrated into the E. coli chromosome by Integrase-mediated recombination (using pIntAssist as helper vector). |
| pZ Integration | Construction of custom E. coli host strains for inducible or constitutive overproduction of recombinant proteins from chromosomally integrated expression units. |
Vectors for chromosomal integration: pZS43MCS-Int (pZS43MCS-Int.pdf) pZS43MCSlaci-Int (pZS43MCSlaci-Int.pdf) pIntAssist |
Vectors carry promoter PA1lacO-1+MCS either for constitutive (pZS43MCS-Int) or for IPTG inducible expression (pZS43MCSlaci-Int). • Resistance module: Spectinomycin • Replication module: pSC101 All vectors can be propagated episomally or can be integrated into the E. coli chromosome by Integrase-mediated recombination (using pIntAssist as helper vector). |